Twilight House is a Battle Stage in Mario Kart DS that has 9 empty rooms and an very thin floor around the house with item boxes on the corners. The stage is floating above an abyss with a forest-like appearance. It is easy to fall off the course when driving around the edges. The rooms of the house are surrounded by fences. Visually, the background suggests it is haunted. Mission 3-8 takes place here. Mario has to collect 20 coins around the house. The stage's name may have been taken from Twilight Town from Paper Mario: The Thousand-Year Door.
The stage returns in Mario Kart Wii as one of the retro battle stages. It's still exactly the same as the DS (you can still get lost on this stage), only with better graphics.