Choco Island 1 & 2These Piranha Plants first appeared in Choco Island 1 and 2. There are scattered around the track and even some in the mud. They snap their petals and don't move around.
Piranha Plants then appear in Mario Kart: Double Dash!! Mario Circuit, where there are a few of them on each side when turning a U turn, and they will bite you if you are too close to them. They also appear at the very end of the course. They acutally snap out at you, blocking your path. They also appear in Yoshi Circuit at more points, and are harder to avoid. Fake metal piranha plants appear in Waluigi Stadium.
They appear in Mario Circuit in this game as well! They appear in pipes (as in Mario Kart: Double Dash!!) and on the ground (as in Mario Kart 64). There are two that actually breath fireballs at you.
They appear in the New Course Piranha Plant Slide and for the first time they have their own course.
They reappear in 3DS Piranha Plant Slide, and also are now an obtainable item.
Fake statued plants appeared in Falco Stadium. They seem to be made of wood and come out of pipes that are on their side in the halfpipe.